Journal: PLoS ONE

Article Title: Whole Genome Complete Resequencing of Bacillus subtilis Natto by Combining Long Reads with High-Quality Short Reads

doi: 10.1371/journal.pone.0109999

Figure Lengend Snippet: Summary of sequence reads.

Article Snippet: We resequenced the BEST195 genome using a PacBio RS sequencer, and we successfully obtained a complete genome sequence from one scaffold without any gaps, and we also applied Illumina MiSeq short reads to enhance quality.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Whole Genome Complete Resequencing of Bacillus subtilis Natto by Combining Long Reads with High-Quality Short Reads

doi: 10.1371/journal.pone.0109999

Figure Lengend Snippet: Assembly results with PacBio subreads and Illumina MiSeq reads.

Article Snippet: We resequenced the BEST195 genome using a PacBio RS sequencer, and we successfully obtained a complete genome sequence from one scaffold without any gaps, and we also applied Illumina MiSeq short reads to enhance quality.

Techniques:

Journal: PLoS ONE

Article Title: Whole Genome Complete Resequencing of Bacillus subtilis Natto by Combining Long Reads with High-Quality Short Reads

doi: 10.1371/journal.pone.0109999

Figure Lengend Snippet: BSNT_06684 and BSNT_06685 are orthologous to BSU03480 and BSU03490, respectively. The upper part of this figure shows an anchor alignment within the new genome using Murasaki, a multiple genome comparison program , and the bottom part displays the mapping results of Illumina GAII reads, MiSeq reads, and PacBio reads.

Article Snippet: We resequenced the BEST195 genome using a PacBio RS sequencer, and we successfully obtained a complete genome sequence from one scaffold without any gaps, and we also applied Illumina MiSeq short reads to enhance quality.

Techniques: Comparison

Journal: PLoS ONE

Article Title: Whole Genome Complete Resequencing of Bacillus subtilis Natto by Combining Long Reads with High-Quality Short Reads

doi: 10.1371/journal.pone.0109999

Figure Lengend Snippet: The region correspond to 4,003,725 bp to 4,007,944 bp in the previous genome. The upper part of this figure shows the mapping results of Illumina GAII reads, MiSeq reads, and PacBio reads. The bottom part displays an anchor alignment within the new genome using Murasaki, a multiple genome comparison program . For the anchor alignment, blue regions represent aligned anchors, which means that the same subsequences are present in other positions of the genome. For the mapping results, reads coloured with white were mapped with mapping quality zero; that is, each read is not uniquely mapped to one position. Compared with the mapping results of Illumina reads, blue regions in the anchor alignment corresponded to positions where white reads were mapped. Additionally, both ends of the gap region include transposases with repetitive sequences.

Article Snippet: We resequenced the BEST195 genome using a PacBio RS sequencer, and we successfully obtained a complete genome sequence from one scaffold without any gaps, and we also applied Illumina MiSeq short reads to enhance quality.

Techniques: Comparison

Journal: BMC Genomics

Article Title: Alignment-free supervised classification of metagenomes by recursive SVM

doi: 10.1186/1471-2164-14-641

Figure Lengend Snippet: Summary of the NGS short reads data of the tree genome data

Article Snippet: The data are NGS short reads of 51 bp long sequenced by the Illumina platform and there is no complete genome sequence.

Techniques: